A dynamic interface between vacuoles and mitochondria in yeast

Yael Elbaz-Alon; Eden Rosenfeld-Gur; Vera Shinder; Anthony H. Futerman; Tamar Geiger; Maya Schuldiner

doi:10.1016/j.devcel.2014.06.007

A dynamic interface between vacuoles and mitochondria in yeast

Yael Elbaz-Alon, Eden Rosenfeld-Gur, Vera Shinder, Anthony H. Futerman, Tamar Geiger, Maya Schuldiner

Tel Aviv University, Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.

Original language	English
Pages (from-to)	95-102
Number of pages	8
Journal	Developmental Cell
Volume	30
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2014.06.007
State	Published - 14 Jul 2014

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry,Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2014.06.007

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title = "A dynamic interface between vacuoles and mitochondria in yeast",

abstract = "Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.",

author = "Yael Elbaz-Alon and Eden Rosenfeld-Gur and Vera Shinder and Futerman, \{Anthony H.\} and Tamar Geiger and Maya Schuldiner",

note = "Funding Information: We thank Jodi Nunnari for sharing the MTS-RFP plasmid and Christian Ungermann and Benedikt Westermann for the mBFP plasmid. We thank Oren Elbaz for immense help with the graphic design, Michal Breker and Silvia Chuartzman for technical assistance with the screening system, and Shai Fuchs for technical assistance with the flow cytometer. We thank Oren Schuldiner, Tslil Ast, and Shai Fuchs for critical reading of the manuscript. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study was funded by a European Research Council Starting Grant (260395). Y.E.-A. was the recipient of the Weizmann Institute Dean{\textquoteright}s postdoctoral fellowship. M.S. is an awardee of the EMBO Young Investigator Program, in conjunction with the Israel Ministry of Science, and a recipient of Marie Curie International Reintegration Grant 239224 of the European Union. A.H.F. is the Joseph Meyerhoff Professor of Biochemistry at the Weizmann Institute of Science. T.G. is supported by the Israel Science Foundation.",

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T1 - A dynamic interface between vacuoles and mitochondria in yeast

AU - Elbaz-Alon, Yael

AU - Rosenfeld-Gur, Eden

AU - Shinder, Vera

AU - Futerman, Anthony H.

AU - Geiger, Tamar

AU - Schuldiner, Maya

N1 - Funding Information: We thank Jodi Nunnari for sharing the MTS-RFP plasmid and Christian Ungermann and Benedikt Westermann for the mBFP plasmid. We thank Oren Elbaz for immense help with the graphic design, Michal Breker and Silvia Chuartzman for technical assistance with the screening system, and Shai Fuchs for technical assistance with the flow cytometer. We thank Oren Schuldiner, Tslil Ast, and Shai Fuchs for critical reading of the manuscript. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. This study was funded by a European Research Council Starting Grant (260395). Y.E.-A. was the recipient of the Weizmann Institute Dean’s postdoctoral fellowship. M.S. is an awardee of the EMBO Young Investigator Program, in conjunction with the Israel Ministry of Science, and a recipient of Marie Curie International Reintegration Grant 239224 of the European Union. A.H.F. is the Joseph Meyerhoff Professor of Biochemistry at the Weizmann Institute of Science. T.G. is supported by the Israel Science Foundation.

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N2 - Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.

AB - Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure(ERMES) was identified as an important yet nonessential contact for such transport. Using a high-contentscreen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (va. cuo. le and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other,and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.

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DO - 10.1016/j.devcel.2014.06.007

M3 - مقالة

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SN - 1534-5807

VL - 30

SP - 95

EP - 102

JO - Developmental Cell

JF - Developmental Cell

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A dynamic interface between vacuoles and mitochondria in yeast

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