TY - JOUR
T1 - A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytes
AU - Zhang, Hong
AU - Gregorio, Josh D.
AU - Iwahori, Toru
AU - Zhang, Xiangyue
AU - Choi, Okmi
AU - Tolentino, Lorna L.
AU - Prestwood, Tyler
AU - Carmi, Yaron
AU - Engleman, Edgar G.
N1 - Publisher Copyright: © 2017, National Academy of Sciences. All rights reserved.
PY - 2017/2/21
Y1 - 2017/2/21
N2 - Plasmacytoid dendritic cells (pDCs) are known mainly for their secretion of type I IFN upon viral encounter. We describe a CD2hiCD5+CD81+ pDC subset, distinguished by prominent dendrites and a mature phenotype, in human blood, bone marrow, and tonsil, which can be generated from CD34+ progenitors. These CD2hiCD5+CD81+ cells express classical pDC markers, as well as the toll-like receptors that enable conventional pDCs to respond to viral infection. However, their gene expression profile is distinct, and they produce little or no type I IFN upon stimulation with CpG oligonucleotides, likely due to their diminished expression of IFN regulatory factor 7. A similar population of CD5+CD81+ pDCs is present in mice and also does not produce type I IFN after CpG stimulation. In contrast to conventional CD5-CD81- pDCs, human CD5+CD81+ pDCs are potent stimulators of B-cell activation and antibody production and strong inducers of T-cell proliferation and Treg formation. These findings reveal the presence of a discrete pDC population that does not produce type I IFN and yet mediates important immune functions previously attributed to all pDCs.
AB - Plasmacytoid dendritic cells (pDCs) are known mainly for their secretion of type I IFN upon viral encounter. We describe a CD2hiCD5+CD81+ pDC subset, distinguished by prominent dendrites and a mature phenotype, in human blood, bone marrow, and tonsil, which can be generated from CD34+ progenitors. These CD2hiCD5+CD81+ cells express classical pDC markers, as well as the toll-like receptors that enable conventional pDCs to respond to viral infection. However, their gene expression profile is distinct, and they produce little or no type I IFN upon stimulation with CpG oligonucleotides, likely due to their diminished expression of IFN regulatory factor 7. A similar population of CD5+CD81+ pDCs is present in mice and also does not produce type I IFN after CpG stimulation. In contrast to conventional CD5-CD81- pDCs, human CD5+CD81+ pDCs are potent stimulators of B-cell activation and antibody production and strong inducers of T-cell proliferation and Treg formation. These findings reveal the presence of a discrete pDC population that does not produce type I IFN and yet mediates important immune functions previously attributed to all pDCs.
KW - CD2
KW - CD5
KW - CD81
KW - Plasmacytoid dendritic cells
KW - Type I IFN
UR - http://www.scopus.com/inward/record.url?scp=85013293350&partnerID=8YFLogxK
U2 - https://doi.org/10.1073/pnas.1610630114
DO - https://doi.org/10.1073/pnas.1610630114
M3 - Article
C2 - 28167780
SN - 0027-8424
VL - 114
SP - 1988
EP - 1993
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -