A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids

Adam Jozwiak; Sayantan Panda; Ryota Akiyama; Ayano Yoneda; Naoyuki Umemoto; Kazuki Saito; Shuhei Yasumoto; Toshiya Muranaka; Sachin A. Gharat; Yana Kazachkova; Yonghui Dong; Shlomy Arava; Inna Goliand; Reinat Nevo; Ilana Rogachev; Sagit Meir; Masaharu Mizutani; Asaph Aharoni

doi:https://doi.org/10.1126/science.adq5721

A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids

Adam Jozwiak, Sayantan Panda, Ryota Akiyama, Ayano Yoneda, Naoyuki Umemoto, Kazuki Saito, Shuhei Yasumoto, Toshiya Muranaka, Sachin A. Gharat, Yana Kazachkova, Yonghui Dong, Shlomy Arava, Inna Goliand, Reinat Nevo, Ilana Rogachev, Sagit Meir, Masaharu Mizutani, Asaph Aharoni

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Decades of research on the infamous antinutritional steroidal glycoalkaloids (SGAs) in Solanaceae plants have provided deep insights into their metabolism and roles. However, engineering SGAs in heterologous hosts has remained a challenge. We discovered that a protein evolved from the machinery involved in building plant cell walls is the crucial link in the biosynthesis of SGAs. We show that cellulose synthase-like M [GLYCOALKALOID METABOLISM15 (GAME15)] functions both as a cholesterol glucuronosyltransferase and a scaffold protein. Silencing GAME15 depletes SGAs, which makes plants more vulnerable to pests. Our findings illuminate plant evolutionary adaptations that balance chemical defense and self-toxicity and open possibilities for producing steroidal compounds in heterologous systems for food, cosmetics, and pharmaceuticals.

Original language	English
Article number	eadq5721
Pages (from-to)	eadq5721
Number of pages	15
Journal	Science
Volume	386
Issue number	6728
DOIs	https://doi.org/10.1126/science.adq5721
State	Published - 20 Dec 2024

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Jozwiak, A., Panda, S., Akiyama, R., Yoneda, A., Umemoto, N., Saito, K., Yasumoto, S., Muranaka, T., Gharat, S. A., Kazachkova, Y., Dong, Y., Arava, S., Goliand, I., Nevo, R., Rogachev, I., Meir, S., Mizutani, M., & Aharoni, A. (2024). A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids. Science, 386(6728), eadq5721. Article eadq5721. https://doi.org/10.1126/science.adq5721

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title = "A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids",

abstract = "Decades of research on the infamous antinutritional steroidal glycoalkaloids (SGAs) in Solanaceae plants have provided deep insights into their metabolism and roles. However, engineering SGAs in heterologous hosts has remained a challenge. We discovered that a protein evolved from the machinery involved in building plant cell walls is the crucial link in the biosynthesis of SGAs. We show that cellulose synthase-like M [GLYCOALKALOID METABOLISM15 (GAME15)] functions both as a cholesterol glucuronosyltransferase and a scaffold protein. Silencing GAME15 depletes SGAs, which makes plants more vulnerable to pests. Our findings illuminate plant evolutionary adaptations that balance chemical defense and self-toxicity and open possibilities for producing steroidal compounds in heterologous systems for food, cosmetics, and pharmaceuticals.",

author = "Adam Jozwiak and Sayantan Panda and Ryota Akiyama and Ayano Yoneda and Naoyuki Umemoto and Kazuki Saito and Shuhei Yasumoto and Toshiya Muranaka and Gharat, {Sachin A.} and Yana Kazachkova and Yonghui Dong and Shlomy Arava and Inna Goliand and Reinat Nevo and Ilana Rogachev and Sagit Meir and Masaharu Mizutani and Asaph Aharoni",

year = "2024",

month = dec,

day = "20",

doi = "https://doi.org/10.1126/science.adq5721",

language = "الإنجليزيّة",

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Jozwiak, A, Panda, S, Akiyama, R, Yoneda, A, Umemoto, N, Saito, K, Yasumoto, S, Muranaka, T, Gharat, SA, Kazachkova, Y, Dong, Y, Arava, S, Goliand, I, Nevo, R, Rogachev, I, Meir, S, Mizutani, M & Aharoni, A 2024, 'A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids', Science, vol. 386, no. 6728, eadq5721, pp. eadq5721. https://doi.org/10.1126/science.adq5721

TY - JOUR

T1 - A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids

AU - Jozwiak, Adam

AU - Panda, Sayantan

AU - Akiyama, Ryota

AU - Yoneda, Ayano

AU - Umemoto, Naoyuki

AU - Saito, Kazuki

AU - Yasumoto, Shuhei

AU - Muranaka, Toshiya

AU - Gharat, Sachin A.

AU - Kazachkova, Yana

AU - Dong, Yonghui

AU - Arava, Shlomy

AU - Goliand, Inna

AU - Nevo, Reinat

AU - Rogachev, Ilana

AU - Meir, Sagit

AU - Mizutani, Masaharu

AU - Aharoni, Asaph

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Decades of research on the infamous antinutritional steroidal glycoalkaloids (SGAs) in Solanaceae plants have provided deep insights into their metabolism and roles. However, engineering SGAs in heterologous hosts has remained a challenge. We discovered that a protein evolved from the machinery involved in building plant cell walls is the crucial link in the biosynthesis of SGAs. We show that cellulose synthase-like M [GLYCOALKALOID METABOLISM15 (GAME15)] functions both as a cholesterol glucuronosyltransferase and a scaffold protein. Silencing GAME15 depletes SGAs, which makes plants more vulnerable to pests. Our findings illuminate plant evolutionary adaptations that balance chemical defense and self-toxicity and open possibilities for producing steroidal compounds in heterologous systems for food, cosmetics, and pharmaceuticals.

AB - Decades of research on the infamous antinutritional steroidal glycoalkaloids (SGAs) in Solanaceae plants have provided deep insights into their metabolism and roles. However, engineering SGAs in heterologous hosts has remained a challenge. We discovered that a protein evolved from the machinery involved in building plant cell walls is the crucial link in the biosynthesis of SGAs. We show that cellulose synthase-like M [GLYCOALKALOID METABOLISM15 (GAME15)] functions both as a cholesterol glucuronosyltransferase and a scaffold protein. Silencing GAME15 depletes SGAs, which makes plants more vulnerable to pests. Our findings illuminate plant evolutionary adaptations that balance chemical defense and self-toxicity and open possibilities for producing steroidal compounds in heterologous systems for food, cosmetics, and pharmaceuticals.

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U2 - https://doi.org/10.1126/science.adq5721

DO - https://doi.org/10.1126/science.adq5721

M3 - مقالة

C2 - 39700293

SN - 0036-8075

VL - 386

SP - eadq5721

JO - Science

JF - Science

IS - 6728

M1 - eadq5721

ER -

A cellulose synthase-like protein governs the biosynthesis of Solanum alkaloids

Abstract

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