A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

I. Kviatkovski; T. Yarnitzky; S. Shushan; O. Schwartz-Harari; R. Nir-Paz; Y. Helman

doi:https://doi.org/10.1039/c8cc03540g

A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

I. Kviatkovski, T. Yarnitzky, S. Shushan, O. Schwartz-Harari, R. Nir-Paz, Y. Helman

The Hebrew University of Jerusalem, Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

Original language	English
Pages (from-to)	9218-9221
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	66
Early online date	26 Jul 2018
DOIs	https://doi.org/10.1039/c8cc03540g
State	Published - 14 Aug 2018

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1039/c8cc03540g

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Kviatkovski, I., Yarnitzky, T., Shushan, S., Schwartz-Harari, O., Nir-Paz, R., & Helman, Y. (2018). A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone. Chemical Communications, 54(66), 9218-9221. Advance online publication. https://doi.org/10.1039/c8cc03540g

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title = "A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone",

abstract = "2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.",

author = "I. Kviatkovski and T. Yarnitzky and S. Shushan and O. Schwartz-Harari and R. Nir-Paz and Y. Helman",

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doi = "https://doi.org/10.1039/c8cc03540g",

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T1 - A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

AU - Kviatkovski, I.

AU - Yarnitzky, T.

AU - Shushan, S.

AU - Schwartz-Harari, O.

AU - Nir-Paz, R.

AU - Helman, Y.

PY - 2018/8/14

Y1 - 2018/8/14

N2 - 2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

AB - 2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

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U2 - https://doi.org/10.1039/c8cc03540g

DO - https://doi.org/10.1039/c8cc03540g

M3 - مقالة

C2 - 30065984

SN - 1359-7345

VL - 54

SP - 9218

EP - 9221

JO - Chemical Communications

JF - Chemical Communications

IS - 66

ER -

A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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