@inproceedings{d806124f3b004133832a7dbd307064e2,
title = "16-Ch time-resolved single-molecule spectroscopy using line excitation",
abstract = "Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.",
keywords = "Fluorescence, High-throughput, SPAD array, Single-molecule, TCSPC",
author = "Antonino Ingargiola and Pietro Peronio and Eitan Lerner and Angelo Gulinatti and Ivan Rech and Massimo Ghioni and Shimon Weiss and Xavier Michalet",
note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Single Molecule Spectroscopy and Superresolution Imaging X ; Conference date: 28-01-2017 Through 29-01-2017",
year = "2017",
month = jan,
day = "28",
doi = "10.1117/12.2256367",
language = "الإنجليزيّة",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Gryczynski, {Zygmunt Karol} and Jorg Enderlein and Ingo Gregor and Felix Koberling and Rainer Erdmann",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging X",
address = "الولايات المتّحدة",
}