• Institute of Nanotechnology and Advanced Materials, Building 206, 4th floor, Room 438

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Personal profile

Research interests

Prof. Shav-Tal received his third degree from the Weizmann Institute of Science and completed his post-doctorate at the Einstein School of Medicine in New York. Then, in 2005, he joined the Faculty of Life Sciences and the research staff at the Institute of Nanotechnology and Advanced Materials of Bar-Ilan University.

Prof. Shav-Tal has published over 100 articles and won many awards and research grants, including prestigious research grants from the ERC and the NIH.

Prof. Shav-Tal's research group focuses on the activity of genes within living cells and during the life of the RNA molecule. The uniqueness of the research is in the ability to follow the activity of a single gene in a living cell in real-time, and the RNA molecules formed from active genes through fluorescent labeling of the mRNA molecules and tracking them with the help of new and sensitive microscopic technologies.

Our research focuses on the gene expression pathway, and specifically on mRNA dynamics in living cell systems. We study dynamic cell processes on the single-molecule, single-gene and single-cell level using time-lapse fluorescent microscopy and subsequent kinetic analysis.
The primary goals of our research are to understand how genes switch "on" and "off" in normal cells and in cancer cells, how quickly are mRNAs transcribed, the kinetics of the transcription process in vivo, and their travels and destinations as they translocate within the cell. The major topics of interest in our group:

Cancer and Gene Expression

We follow gene expression in real-time by applying a variety of fluorescent tags to genes, mRNAs and proteins, and have developed a number of cell systems in which gene expression, or mRNA transcription, can be examined and quantified.

We are able to observe single genes in living cells and to quantify the action of a gene as it unfolds before our eyes. Using this approach, we can monitor the influence of promoter regions and transcription factors, on the activity of an oncogene in living cells, thereby elucidating the over-expression pathway in cancerous cells.

As the process of transcription transpires, the pre-mRNA undergoes a number of processing events such as capping, splicing and polyadenylation. Since these processes occur co-transcriptionally, it is important to determine whether they affect transcription kinetics. We are examining the real-time kinetics of the co-transcriptional process of pre-mRNA splicing using live cell imaging techniques (FRAP, photoactivation and FCS) followed by kinetic modeling for analysis of the kinetic data.

RNA Export

In order to understand how genetic information disseminates from the cell nucleus into the cytoplasm it is essential to determine the mechanisms of mRNA mobility in cells. We have been following the dynamics of mRNA nucleoplasmic translocation, as well as mRNA export in vivo. We are interested in analyzing different elements that control the export pathway, using inhibitors and knock down of specific elements considered necessary for these processes. Our analysis performed by applying single molecule approaches that allow us to quantify the interactions of molecules passing through the nuclear pore complex. We also perform screening of small molecule libraries using high-content microscopy in search of new inhibitors of the gene expression pathway.

Cytoplasmic mRNA

Cytoplasmic mRNAs can be translated by ribosomes, stored in granules, or degraded by a variety of surveillance mechanisms. A group of structures involved in mRNA storage and decay are cytoplasmic P-bodies. We are interested in understanding the dynamics of cytoplasmic P-bodies in living cells in relation to mRNA kinetics. Imaging the "mRNA localization" process in real-time will assist in revealing the fate of mRNAs in cells.

 

Education/Academic qualification

PhD

Jan 1995Jan 2000

Award Date: 1 Jan 2000

Master

Oct 1993Jun 1995

Award Date: 30 Jun 1995

Bachelor, Bar-Ilan University

Oct 1990Jun 1993

Award Date: 30 Jun 1993

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