Long-held to be a post-translational modification unique to Eukarya, it is now clear that Bacteria and Archaea also perform N-glycosylation, the covalent linkage of glycans to select asparagine residues of target proteins. However, whereas such protein processing is restricted to a limited number of bacterial groups, N-glycosylation is an almost universal event in the Archaea. Work in our lab seeks to better understand the archaeal version of this universal post-translational modification using bioinformatics, genetic, biochemical, and structural approaches.