SETDB1 regulates microtubule dynamics

Rosari Hernandez-Vicens, Jagreeti Singh, Nomi Pernicone, Tamar Listovsky, Gabi Gerlitz

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

ملخص

Objectives: SETDB1 is a methyltransferase responsible for the methylation of histone H3-lysine-9, which is mainly related to heterochromatin formation. SETDB1 is overexpressed in various cancer types and is associated with an aggressive phenotype. In agreement with its activity, it mainly exhibits a nuclear localization; however, in several cell types a cytoplasmic localization was reported. Here we looked for cytoplasmic functions of SETDB1. Methods: SETDB1 association with microtubules was detected by immunofluorescence and co-sedimentation. Microtubule dynamics were analysed during recovery from nocodazole treatment and by tracking microtubule plus-ends in live cells. Live cell imaging was used to study mitotic kinetics and protein–protein interaction was identified by co-immunoprecipitation. Results: SETDB1 co-sedimented with microtubules and partially colocalized with microtubules. SETDB1 partial silencing led to faster polymerization and reduced rate of catastrophe events of microtubules in parallel to reduced proliferation rate and slower mitotic kinetics. Interestingly, over-expression of either wild-type or catalytic dead SETDB1 altered microtubule polymerization rate to the same extent, suggesting that SETDB1 may affect microtubule dynamics by a methylation-independent mechanism. Moreover, SETDB1 co-immunoprecipitated with HDAC6 and tubulin acetylation levels were increased upon silencing of SETDB1. Conclusions: Taken together, our study suggests a model in which SETDB1 affects microtubule dynamics by interacting with both microtubules and HDAC6 to enhance tubulin deacetylation. Overall, our results suggest a novel cytoplasmic role for SETDB1 in the regulation of microtubule dynamics.

اللغة الأصليةالإنجليزيّة
رقم المقالe13348
الصفحات (من إلى)e13348
دوريةCell Proliferation
مستوى الصوت55
رقم الإصدار12
تاريخ مبكر على الإنترنت3 نوفمبر 2022
المعرِّفات الرقمية للأشياء
حالة النشرنَشْر مسبق في الإنترنت - 3 نوفمبر 2022

All Science Journal Classification (ASJC) codes

  • !!Cell Biology

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