New host factors important for respiratory syncytial virus (RSV) replication revealed by a novel microfluidics screen for interactors of matrix (M) protein

Samar Hamad, Leon Caly, Eran Bacharach, David A. Jans, Doron Gerber, Monika Bajorek

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

ملخص

Although human respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and pneumonia in infants and elderly worldwide, there is no licensed RSV vaccine or effective drug treatment available. The RSV Matrix protein plays key roles in virus life cycle, being found in the nucleus early in infection in a transcriptional inhibitory role, and later localizing in viral inclusion bodies before coordinating viral assembly and budding at the plasma membrane. In this study, we used a novel, high throughput microfluidics platform and custom human open reading frame library to identify novel host cell binding partners of RSV matrix. Novel interactors identified included proteins involved in host transcription regulation, the innate immunity response, cytoskeletal regulation, membrane remodeling, and cellular trafficking. A number of these interactions were confirmed by immunoprecipitation and cellular colocalization approaches. Importantly, the physiological significance of matrix interaction with the actin-binding protein cofilin 1, caveolae protein Caveolin 2, and the zinc finger protein ZNF502 was confirmed. siRNA knockdown of the host protein levels resulted in reduced RSV virus production in infected cells. These results have important implications for future antiviral strategies aimed at targets of RSV matrix in the host cell.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)532-543
عدد الصفحات12
دوريةMolecular and Cellular Proteomics
مستوى الصوت14
رقم الإصدار3
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 1 مارس 2015

All Science Journal Classification (ASJC) codes

  • !!Analytical Chemistry
  • !!Biochemistry
  • !!Molecular Biology

بصمة

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