Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells

Michal Rabani, Joshua Z. Levin, Lin Fan, Xian Adiconis, Raktima Raychowdhury, Manuel Garber, Andreas Gnirke, Chad Nusbaum, Nir Hacohen, Nir Friedman, Ido Amit, Aviv Regev

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء


Cellular RNA levels are determined by the interplay of RNA production, processing and degradation. However, because most studies of RNA regulation do not distinguish the separate contributions of these processes, little is known about how they are temporally integrated. Here we combine metabolic labeling of RNA at high temporal resolution with advanced RNA quantification and computational modeling to estimate RNA transcription and degradation rates during the response of mouse dendritic cells to lipopolysaccharide. We find that changes in transcription rates determine the majority of temporal changes in RNA levels, but that changes in degradation rates are important for shaping sharp 'peaked' responses. We used sequencing of the newly transcribed RNA population to estimate temporally constant RNA processing and degradation rates genome wide. Degradation rates vary significantly between genes and contribute to the observed differences in the dynamic response. Certain transcripts, including those encoding cytokines and transcription factors, mature faster. Our study provides a quantitative approach to study the integrative process of RNA regulation.

اللغة الأصليةإنجليزيّة أمريكيّة
الصفحات (من إلى)436-442
عدد الصفحات7
دوريةNature biotechnology
مستوى الصوت29
رقم الإصدار5
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - مايو 2011

All Science Journal Classification (ASJC) codes

  • !!Applied Microbiology and Biotechnology
  • !!Bioengineering
  • !!Molecular Medicine
  • !!Biotechnology
  • !!Biomedical Engineering


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