FIE, a nuclear PRC2 protein, forms cytoplasmic complexes in Arabidopsis thaliana

Moran Oliva, Yana Butenko, Tzung-Fu Hsieh, Ofir Hakim, Aviva Katz, Nechama I. Smorodinsky, Daphna Michaeli, Robert L. Fischer, Nir Ohad

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

ملخص

FIE, a WD-40 subunit of the Arabidopsis PRC2 complexes known to take part in H3K27 methylation of nuclear chromatin, is also localized in cytoplasmic complexes.The core subunit FIE is crucial for PRC2 function throughout the plant life cycle, yet accurate information on its spatial and temporal localization was absent. This study focused on identifying FIE accumulation patterns, using microscopy and biochemical approaches. Analysing endogenous FIE and transgenic gFIE-green fluorescent protein fusion protein (gFIE-GFP) showed that FIE accumulates in the nuclei of every cell type examined. Interestingly, gFIE-GFP, as well as the endogenous FIE, also localized to the cytoplasm in all examined tissues. In both vegetative and reproductive organs, FIE formed cytoplasmic high-molecular-mass complexes, in parallel to the nuclear PRC2 complexes. Moreover, size-exclusion chromatography and bimolecular fluorescence complementation assays indicated that in inflorescences FIE formed a cytoplasmic complex with MEA, a PRC2 histone methyltransferase subunit. In contrast, CLF and SWN histone methyltransferases were strictly nuclear. Presence of PRC2 subunits in cytoplasmic complexes has not been previously described in plants. Our findings are in agreement with accumulating evidence demonstrating cytoplasmic localization and function of PcGs in metazoa. The cytosolic accumulation of PRC2 components in plants supports the model that PcGs have alternative non-nuclear functions that go beyond chromatin methylation.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)6111-6123
عدد الصفحات13
دوريةJournal of Experimental Botany
مستوى الصوت67
رقم الإصدار21
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 1 نوفمبر 2016

All Science Journal Classification (ASJC) codes

  • !!Physiology
  • !!Plant Science

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