Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection

Noa Cohen, Pooja Sabhachandani, Alexander Golberg, Tania Konry

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

ملخص

In this study we describe a simple lab-on-a-chip (LOC) biosensor approach utilizing well mixed microfluidic device and a microsphere-based assay capable of performing near real-time diagnostics of clinically relevant analytes such cytokines and antibodies. We were able to overcome the adsorption kinetics reaction rate-limiting mechanism, which is diffusion-controlled in standard immunoassays, by introducing the microsphere-based assay into well-mixed yet simple microfluidic device with turbulent flow profiles in the reaction regions. The integrated microsphere-based LOC device performs dynamic detection of the analyte in minimal amount of biological specimen by continuously sampling micro-liter volumes of sample per minute to detect dynamic changes in target analyte concentration. Furthermore we developed a mathematical model for the well-mixed reaction to describe the near real time detection mechanism observed in the developed LOC method. To demonstrate the specificity and sensitivity of the developed real time monitoring LOC approach, we applied the device for clinically relevant analytes: Tumor Necrosis Factor (TNF)-α cytokine and its clinically used inhibitor, anti-TNF-α antibody. Based on the reported results herein, the developed LOC device provides continuous sensitive and specific near real-time monitoring method for analytes such as cytokines and antibodies, reduces reagent volumes by nearly three orders of magnitude as well as eliminates the washing steps required by standard immunoassays.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)454-460
عدد الصفحات7
دوريةBiosensors and Bioelectronics
مستوى الصوت66
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 5 أبريل 2015
منشور خارجيًانعم

All Science Journal Classification (ASJC) codes

  • !!Biotechnology
  • !!Biophysics
  • !!Biomedical Engineering
  • !!Electrochemistry

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