Analysis of microRNA Regulation in Single Cells

Wendao Liu, Noam Shomron

نتاج البحث: فصل من :كتاب / تقرير / مؤتمرفصلمراجعة النظراء

ملخص

MicroRNAs (miRNAs) regulate gene expression by binding to mRNAs. Consequently, they reduce target gene expression levels and expression variability, also known as “noise.” Single-cell RNA sequencing (scRNA-seq) technology has been used to study miRNA and mRNA expression in single cells, and has demonstrated its strength in quantifying cell-to-cell variation. Here we describe how to investigate miRNA regulation using data with both mRNA and miRNA expression in single cell format. We show that miRNAs reduce the expression levels and also expression noise of target genes in single cells. Finally, we also discuss potential improvements in experimental design and computational analysis of scRNA-seq in order to reduce or partition the technical noise.

اللغة الأصليةالإنجليزيّة
عنوان منشور المضيفMethods in Molecular Biology
الصفحات339-354
عدد الصفحات16
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 2021

سلسلة المنشورات

الاسمMethods in Molecular Biology
مستوى الصوت2243

All Science Journal Classification (ASJC) codes

  • !!Molecular Biology
  • !!Genetics

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