Abortive intussusceptive angiogenesis causes multi-cavernous vascular malformations

Wenqing Li, Virginia Tran, Iftach Shaked, Belinda Xue, Thomas Moore, Rhonda Lightle, David Kleinfeld, Issam A. Awad, Mark H. Ginsberg

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

ملخص

Mosaic inactivation of CCM2 in humans causes cerebral cavernous malformations (CCMs) containing adjacent dilated blood-filled multi-cavernous lesions. We used CRISPR-Cas9 mutagenesis to induce mosaic inactivation of zebrafish ccm2 resulting in a novel lethal multi-cavernous lesion in the embryonic caudal venous plexus (CVP) caused by obstruction of blood flow by intraluminal pillars. These pillars mimic those that mediate intussusceptive angiogenesis; however, in contrast to the normal process, the pillars failed to fuse to split the pre-existing vessel in two. Abortive intussusceptive angiogenesis stemmed from mosaic inactivation of ccm2 leading to patchy klf2a overexpression and resultant aberrant flow signaling. Surviving adult fish manifested histologically typical hemorrhagic CCM. Formation of mammalian CCM requires the flow-regulated transcription factor KLF2; fish CCM and the embryonic CVP lesion failed to form in klf2a null fish indicating a common pathogenesis with the mammalian lesion. These studies describe a zebrafish CCM model and establish a mechanism that can explain the formation of characteristic multi-cavernous lesions.

اللغة الأصليةالإنجليزيّة
رقم المقالe62155
دوريةeLife
مستوى الصوت10
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - مايو 2021
منشور خارجيًانعم

All Science Journal Classification (ASJC) codes

  • !!General Immunology and Microbiology
  • !!General Biochemistry, Genetics and Molecular Biology
  • !!General Neuroscience

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